Physiologic Response Evaluation of Human Foetal Osteoblast Cells within Engineered 3D-Printed Polylactic Acid Scaffolds

Physiologic Response Evaluation of Human Foetal Osteoblast Cells within Engineered 3D-Printed Polylactic Acid Scaffolds

Blog Article

Large bone defect treatments have always been one of the important challenges in clinical practice and created a huge demand for here more efficacious regenerative approaches.The bone tissue engineering (BTE) approach offered a new alternative to conventional bone grafts, addressing all clinical needs.Over the past years, BTE research is focused on the study and realisation of new biomaterials, including 3D-printed supports to improve mechanical, structural and biological properties.

Among these, polylactic acid (PLA) scaffolds have been considered the most promising biomaterials due to their good biocompatibility, non-toxic biodegradability and bioresorbability.In this work, we evaluated the physiological response of human foetal osteoblast cells (hFOB), in terms of cell proliferation and osteogenic differentiation, within oxygen plasma treated 3D-printed PLA scaffolds, obtained by fused deposition modelling (FDM).A mechanical simulation to predict their behaviour to traction, flexural or torque solicitations was performed.

We found that: 1.hFOB cells adhere and grow on scaffold surfaces; 2.hFOB grown on oxygen plasma treated PLA scaffolds (PLA_PT) show an improvement of cell adhesion and proliferation, compared to not-plasma treated scaffolds (PLA_NT); 3.

Over time, hFOB penetrate read more along strands, differentiate, and form a fibrous matrix, tissue-like; 4.3D-printed PLA scaffolds have good mechanical behaviour in each analysed configuration.These findings suggest that 3D-printed PLA scaffolds could represent promising biomaterials for medical implantable devices in the orthopaedic field.